Oral Glucose Tolerance Test (OGTT)

At one week before the end of treatment, all animals were fasted for 12 hours. Initially, the baseline blood was collected to assess basal glucose concentration from the tail vein. Then a glucose solution (1.4 g/kg of body weight) was administrated by gavage. Blood samples were collected again after 15, 30, 45, 60 and 120 minutes to obtain the glycemic curve. The Homeostasis Model Assessment Insulin Resistance (HOMA-IR) was calculated taking into consideration fasting insulin (μU/mL) and fasting glucose (mmol/L), as follows: HOMA-IR = (insulin × glucose)/22.5.