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Nitric oxide (NO) and cell viability measurements
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The Griess assay was performed as described previously to assess the effect of MJ on the secretion of NO by LPS-stimulated BV-2 cells (Spielman et al., 2017). The viability of cells was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay as described by Madeira et al. (2012). For all cell types used, the non-toxic MJ concentration range and incubation times were selected in preliminary experiments.
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