Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Intracellular triglyceride quantification

YB Yuri L. Boteon
LW Lorraine Wallace
AB Amanda P. C. S. Boteon
DM Darius F. Mirza
HM Hynek Mergental
RB Ricky H. Bhogal
SA Simon Afford
request Request a Protocol
ask Ask a question
Favorite

At the end of the incubation period, cells were washed with PBS and harvested with gentle scraping. Intracellular lipids were retrieved using the detergent TERGITOL™ Type NP-40 (NP40S; Sigma-Aldrich) followed by lipase incubation to break down triglycerides into FAs and glycerol. Intracellular triglyceride was measured using a colorimetric assay (ab65336; Abcam, Cambridge, MA, USA) based on a reaction of glycerol oxidation producing colour. The concentration of triglycerides was normalised to control and expressed as nmol per million of cells.

Copyright and License information:  ©2018 Boteon et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A