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Cell viability was detected by an MTT assay. In brief, the cells were incubated on a 96-well plate until 80% cell confluence and then subjected to transfection and doxorubicin treatment. After incubation, cell viability was analyzed using a Cell Proliferation Kit 1 (MTT) (Sigma-Aldrich). MTT solution was added to each well and cultured for 4 h. The reaction was stopped, and DMSO was added to dissolve formazan crystals. The final mixture was analyzed using an enzyme-linked immunometric meter at 490 nm.
Copyright and License information: The Author(s). ©2019
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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