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Fluorescence emission values were used to monitor the protein amount. The fluorescence emission values were obtained by a Fluoroskan Ascent microplate fluorometer with an excitation wavelength of 485 nm and an emission wavelength of 538 nm. The protein amount was calculated using the protocol established in [12]. For the experiments comparing mutants’ activity, fluorescence emission values were always measured to ensure equal protein amounts.
Copyright and License information: The Author(s). ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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