In vivo experiments and tumor models

Balb/c mice were used (Charles River Laboratories) for the 4 T1 and EMT6 murine tumor models. For orthotopic implantation of the tumors, 2 × 10⁵ cells were injected into the second left mammary fat pad. For the EO771 tumor model, 1 × 10⁶ cells were implanted into the second left mammary fat pad of C57/Bl6 mice. For treatments, the virus and drug preparations were diluted to the appropriate concentration in a total volume of 100 uL of PBS and injected IP or intratumorally (IT) using insulin syringes (The Stevens Co, Montreal, QC, Canada). All experiments were performed in accordance with the University of Ottawa animal care and veterinary services guidelines.