IB preparation

5 mL fermentation broth samples were centrifuged at 4800 rpm at 4 °C. The supernatant is discarded and the pellet is resuspended to a DCW of about 4 g/L in lysis buffer (100 mM Tris, 10 mM EDTA at pH 7.4). Afterwards the sample was homogenized using a high-pressure homogenizer at 1500 bar for 10 passages (Emulsiflex C3; Avestin, Ottawa, Canada). After centrifugation at 10,000 rpm and 4 °C the supernatant was discarded and the resulting IB pellet was washed twice with ultrapure water and aliquoted into pellets à 2 mL broth, centrifuged (14,000 rpm, 10 min 4 °C) and stored at − 20 °C.