Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
Rad21 expression was analyzed in paraffin sections using rabbit anti‐Rad21 antibodies (1:200 dilution, Abcam) and an immunohistochemistry assay kit (Origene). Positive immunostaining of Rad21 appeared as a brown or tan color in the nucleus or cytoplasm of cancer cells. According to the proportion of positive cells and staining intensity, the expression level was scored. Expression was considered negative when the total score was 0‐2 and positive when the total score was more than 2.
Copyright and License information: ©2018 The Authors. published by John Wiley & Sons Ltd.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
