Detection of Singlet Oxygen Production

1, 3-diphenylisobenzofuran (DPBF) was used to detect the singlet oxygen. 15 μL DPBF acetonitrile solution was added into pristine ICG or FA-IHA NPs solution (1.0 mL, 10 μg/mL) and mixed thoroughly, followed by 5 min of irradiation (808 nm, 1.0 W/cm²). The UV-vis absorption spectra were recorded at different time points, and the decrease rate of absorption at 410 nm is proportional to the singlet oxygen production.