2.4. Measurement of caspase-3 and -7 activities

Using the Caspase-Glo 3/7 assay (Promega), the effect of TQ and Aβ_1–42 on apoptosis was investigated by measuring Caspase 3 and 7 activities. Aβ_1–42 (5 μM) was added to hiPSC-derived cholinergic neurons alone or simultaneously with TQ (100 nM) for 48 h. At the end of the incubation time, assay reagents were prepared according to the manufacturer's instructions and applied to the cells. After 1 h incubation at room temperature, a microplate reader (TECAN) was used to measure luminescence signals.