LPS-Induced Neuroinflammatory Model In Mice

As previously reported, LPS can induce the neuroinflammation in mice (Leite et al., 2016). Firstly, mice were randomly separated into five groups (n = 6–9 of successfully treated rats per group). Mice were intraperitoneally injected with 20 mg/kg, 60 mg/kg, or 100 mg/kg CBL dissolved in the saline or saline alone daily in the 3 consecutive days. On the third day, at 3 h after the administration of CBL or saline in mice, LPS (0.33 mg/kg) dissolved in the saline was intraperitoneally injected according to the body weight of the mice. After 3 h, all the mice were subjected to Open field test to analyze the sickness behavior induced by the LPS. After the behavior test, the blood of the mouse was immediately taken for measurement of pro-inflammatory factors, and the whole cerebral cortex of the mouse was extracted for assessment of inflammatory mediators and related protein expressions.