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Analysis of p38 MAPK by Western blot
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Supernatants of whole spinal cord lysates were resolved on a 3–15% gradient gel by polyacrylamide gel electrophoresis (30 μg protein/lane) and immunoblotted with antibodies to phosphorylated p38 MAPK and unphosphorylated total p38 MAPK (all from Cell Signaling Technology) as described previously.4
Copyright and License information: ©2019 Wang et al.
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