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The cells were placed in the upper matrigel-coated transwell chambers (8 μm pore) (Corning), and the lower chamber containing 10% fetal bovine serum (FBS) media. The invasion assay was performed following the manufacturer’s instructions. The stained cells were obtained under a light microscope (Olympus, Tokyo, Japan).
Copyright and License information: ©2019 The Author(s)
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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