All experiments described in this paper were approved by the 4th Local Ethical Committee based in Warsaw, Poland, and were performed in accordance with Polish governmental regulations (Dz.U.97.111.724), and with the European Community Council Directive of 24 November 1986 (86/609/EEC) and with Directive 2010/63/EU. All surgeries were performed under halothane anaesthesia and all efforts were made to minimize animal suffering and the number of animals used.
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