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4,6-Diamidino-2-Phenylindole Dihydrochloride Staining
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4,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining was used to analyze the cell nuclear morphology. PC9 and H1299 cells were treated with NAT-F for the indicated concentration and time courses. The cells were washed with PBS and fixed with 4% paraformaldehyde for 30 min at RT. After washing with PBS, the cells were stained with DAPI (100 ng/ml) for 15 min in the dark and photographed using a fluorescence microscope (Nikon, Japan).
Copyright and License information: ©2019 Liu, Zhu, Wu, Shen, Lin, Wu, Liu, Tang, Zhou, Sun and Lin
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