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Determination of equilibrium solubility of ETD in water
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An excess amount of ETD was added in a plastic cap screw glass vial containing 10 mL of water. The vial was placed in an incubator, set to shake at 75 rpm at 37 ± 0.5 °C for 24 h, then the vial was allowed to rest for another 24 h at the same temperature, then the content was filtered, diluted appropriately and measured using a UV–Visible spectrophotometer (Shimadzu UV 1800 PC, Shimadzu, Kyoto, Japan) at 278 nm which corresponds to the λmax of the ETD.
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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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