EZ‐Magna ChIP™ Kit (Millipore, Billerica, USA) was used for ChIP‐qPCR assay. The anti‐HMGA2 antibody (8 μg; Abcam, Cambridge, USA) and anti‐IgG antibody (8 μg; Sigma, St. Louis, USA) were used to precipitate the chromatin. The ChIP was performed under the guidance of the manufacturer's instruction. The ChIP‐qPCR primers are listed in Table S2 (Sangon, Shanghai, China).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.