2.5. ChIP‐qPCR assays

SZ Shanshan Zhang
HZ Huibian Zhang
LY Lin Yu
request Request a Protocol
ask Ask a question
Favorite

EZ‐Magna ChIP Kit (Millipore, Billerica, USA) was used for ChIP‐qPCR assay. The anti‐HMGA2 antibody (8 μg; Abcam, Cambridge, USA) and anti‐IgG antibody (8 μg; Sigma, St. Louis, USA) were used to precipitate the chromatin. The ChIP was performed under the guidance of the manufacturer's instruction. The ChIP‐qPCR primers are listed in Table S2 (Sangon, Shanghai, China).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A