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Negative staining transmission electron microscopy (TEM)
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Prepared Aβ1–40 fibrils were sonicated for 60 s in a water bath and a total volume of 3.5 μl of fibrils was applied to 300 mesh formvar/carbon-coated, glow-discharged Ni-grids. After 1.5 min, the grid was washed in distilled water and consequently negatively stained for 15 s with 1.5% uranyl acetate (TAAB, Berks, England). Finally, fibrils were examined under a Talos L120 TEM (FEI) microscope (120 kV) equipped with a Ceta CMOS 4 k × 4 k pixel (FEI) camera supported with the FEI TIA (TEM imaging and analysis).
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Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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