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Encapsulation of fluorescent molecules in PCs

YL Yunju La
JS Jeongeun Song
MJ Moon Gon Jeong
AC Arah Cho
SJ Seon-Mi Jin
EL Eunji Lee
KK Kyoung Taek Kim
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PCs of PEG5503-PS150 were prepared by using a fluorescein sodium salt solution (1 mM in water). After dialysis of the suspension against water for 24 h, the suspension was further purified by the repeated filtration on a centrifugal filter (Amicon, MW cut-off 100 kDa) and exchange of the medium with pure water. This procedure was repeated until the filtrate showed no sign of the presence of free fluorescein on a fluorometer. As a control experiment, the concentrated PCs of PEG5503-PS150 were mixed with a fluorescein solution (1 mM). The resulting mixture was equilibrated under gentle shaking at room temperature for 24 h. This mixture was purified by repeated filtrations on a centrifugal filter and exchange of the medium.

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