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Lactate Dehydrogenase (LDH) Assay for Cell Injury
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Cytotoxicity was determined using an LDH Assay Kit (KeyGen, Nanjing, China), in accordance with the manufacturer’s instructions. Following treatment with hemin for 18 h, the supernatant was collected and transferred to 96-well plates to measure LDH release. The absorbance of each sample was quantified at 450 nm using an automated ELISA reader (SpectraMax® M5, Molecular Devices, United States).
Copyright and License information: ©2018 Zhang, Wang and He.
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