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2.5. Nitric oxide (NO) production levels
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The Griess reaction was used to measure supernatant nitrite concentration as an indicator of NO production. Well supernatant was mixed with an equal volume of Griess reagent (Sigma‐Aldrich, Cat No. G4410) in a 96‐well plate and incubated for 15 minutes. Mixture absorbance was measured at 540 nm using a microplate reader (model 680, Bio‐Rad, Hercules, CA). NO levels were normalized to cell numbers.
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