Patients were asked to rinse with distilled water thoroughly prior to donating saliva and to spit directly into the saliva collection tube. Five milliliters of unstimulated saliva was collected in a collection tube. Samples were diluted to 1:10 with sterile Phosphate Buffered Saline (PBS) and were filter sterilized.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.