Data Analysis

The data were tabulated in Microsoft Excel and are presented as the means and standard deviations for each diverse rapeseed line (parental, F₁, F₂, and BC₁) at both locations. By following the method of Eshed and Zamir (1996), the additive effect (A) and dominance effect (D) of BnFAD2-1 and BnFAD2-2 in F2 populations were calculated as: A = (phenotype value of mutant homozygotes - phenotype value of WT homozygotes)/2; D = (phenotype value of heterozygotes - (phenotype value of mutant homozygotes + phenotype value of WT homozygotes)/2; and the epistasis effect (I) of two genes was: I = [(phenotype value of aabb genotypes + phenotype value of AABB genotypes) - (phenotype value of aa genotypes + phenotype value of bb genotypes)]/2. Comparisons of the oleic acid content of different genotypes were conducted using one-way ANOVA in Graphpad Prism 7 software (Graphpad Prism Software Inc., San Diego, CA, USA). A p-value less than 0.05 was considered significant. A box-and-whiskers plot was made to show the distribution (min, first quartile, median, third quartile, and max) of the oleic acid content under different genotypes in Graphpad Prism 7.