Construction of SLAF-seq Library and High-throughput Sequencing

The current sheep genome (Oar_v4.0) was selected as the reference genome to simulate the restriction enzyme digestion and identify the expected SLAF yield. Hpy166II + EcoRV-HF enzyme was selected for enzymatic digestion (Davey et al., 2013). Furthermore, to evaluate the accuracy of the digestion strategy, Oryza sativa indica ( http://rapdb. dna. affrc. go. JP/) was selected (Li et al., 2009b). Subsequently, data of 126 individuals were used to construct the SLAF library (Kozich et al., 2013) and for sequencing after quality inspection. To evaluate the accuracy of the enzyme test, Nippon Sunshine was selected as the control for sequencing.