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DNA was extracted from frozen tissues or cells using Blood & Cell Culture DNA Mini Kit (Qiagen, #13323). Isolated genomic DNA was bisulfite treated with EZ DNA methylation Gold kit (Zymo Research, #11-335B). The modified DNA was quantified by PCR. One pair of primer (MS-M-F/R) was used to PCR-methylated MGMT promoter. Another pair of primer (MS-U-F/R) was used to PCR unmethylated MGMT promoter. The sequences for PCR primers were listed in supplemental table (Supplementary Table 2).
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