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mRFP-GFP-LC3 mouse fibroblasts

MP Mariana Pavel
MR Maurizio Renna
SP So Jung Park
FM Fiona M. Menzies
TR Thomas Ricketts
JF Jens Füllgrabe
AA Avraham Ashkenazi
RF Rebecca A. Frake
AL Alejandro Carnicer Lombarte
CB Carla F. Bento
KF Kristian Franze
DR David C. Rubinsztein
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The mRFP-GFP-LC3 transgenic mice were created by Dr F. Menzies under the jurisdiction of appropriate Home Office Project and Personal animal licenses and with local Ethics Committee approval, as previously reported in ref. 17.

For primary mRFP-GFP-LC3 mouse fibroblasts, transgenic mice were crossed with C57BL6 mice and pregnant females (E16.5 gestation) were sacrificed and embryos were harvested. The primary mRFP-GFP-LC3 mouse fibroblasts were grown in DMEM (D6546 Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS, F7524 Sigma-Aldrich), 100 U ml−1 penicillin-streptomycin (P0781 Sigma-Aldrich), 2 mM L-glutamine (G7513 Sigma-Aldrich) at 37 °C and 5% CO2, humidified atmosphere.

Copyright and License information: The Author(s) ©2018
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