Sperm motility analysis

To evaluate spermatozoa motility parameters a computer assisted semen analysis - SpermVision™ CASA System (MiniTüb, Tiefenbach, Germany) with Olympus BX 51 phase contrast microscope (Olympus, Tokyo, Japan) was used. Sperm samples were diluted with physiological solution (10 μl) and pipetted into a Makler Counting Chamber (depth 10 μm, Sefi Medical Instruments, Haifa, Israel) and immediately assessed. Within each of the measurement by the CASA system, motility parameters from minimum seven fields of Makler Counting Chamber were analysed and 1000 sperms were evaluated per sample. Motion parameters included the percentage of motile spermatozoa (MOT), the percentage of progressive motility (PROG), distance average path (DAP, μm), distance curved line (DCL, μm), distance straight line (DSL, μm), velocity average path (VAP, μm/s), velocity curved line (VCL, μm/s), velocity straight line (VSL, μm/s), straightness (STR, %), linearity (LIN, %), wobble (WOB, %), amplitude of lateral head displacement (ALH, μm), and beat cross frequency (BCF, Hz).