2.2. Tobacco transformation

Construction of the PHB vector was used for the tobacco (Nicotiana tabacum cv. Xanthi) transformation according to a previous report (Yan et al., 2011) . To begin, 50 mg L–1 hygromycin was used as the selected marker. The transgenic tobacco plants were confirmed through PCR amplification of the aroA gene. The PCR fragments were amplified using specific primers (5-ATGCAGGACTCCCTGACTTTACAG3 ; 5 - TC AG G C G C TC TG G C TG AT T T T TG C C A- 3 ) , resulting in 1287 bp of amplified PCR product (aroA Pantoea sp.). The PCR products were analyzed using 1% agarose gels and electrophoresis.