Measurement of IOP

Goldmann applanation tonometry was used to measure IOP at the baseline (prior to initiation of topical medication) and at every follow-up visit thereafter. For the baseline IOP, the mean of the diurnal IOP measurements taken at 8:30 am, 10:00 am, 11:30 am, 1:00 pm, 2:30 pm, and 4:00 pm was used. Subsequently, all of the patients were administered glaucoma treatment aiming to reduce their baseline IOP by 20% or more. Diurnal IOP variation was determined by calculating the standard deviation (SD) of the 6 IOPs, and the follow-up IOP fluctuation was defined as the SD of the IOPs measured throughout the follow-up period.