Serum Triglyceride Measurement

Serum triglyceride levels in 7, 9, and 12-month-old APOB-100 transgenic (n = 5) and wild-type mice (n = 5) fed on a normal chow diet were measured using a colorimetric assay (Supplementary Table S1). Blood samples were collected through cardiac puncture under terminal anesthesia. After clot formation samples were centrifuged at 4°C, 1000 × g for 10 min, then serum was removed and stored at -80°C until use. Serum triglyceride levels were measured in triplicate using a commercially available enzymatic colorimetric assay kit (Diagnosticum Ltd., Budapest, Hungary) according to the manufacturer’s instructions. Test accuracy was monitored using Standard Lipid Controls (Diagnosticum Ltd., Budapest, Hungary). Absorbance of the produced purple color product was measured at 560 nm using a microplate reader (Multiskan FC, Thermo Scientific, United States). Values were expressed in mmol/liter. Experimental groups, (APOB-100 transgenic mice and wild-type littermates) consisted of 5 animals each.