Antibodies and Chemicals

We used the following primary antibodies: mouse anti-β-actin (Cat No: sc-47778, Santa Cruz Biotechnology, Dallas, TX, United States), mouse anti-p-IκBα (B-9, Cat No: sc-8404, Santa Cruz Biotechnology), mouse anti-IκBα (Cat No: sc-1643, Santa Cruz Biotechnology), rabbit anti-NF-κB (P65, Cat No: sc-8008, Santa Cruz Biotechnology), mouse anti-PCNA (Cat No: sc-56, Santa Cruz Biotechnology), rat anti-mouse CD11b (M1/70, Cat No: ab8878, Abcam, Cambridge, MA, United States), rabbit anti-FAK (Cat No: 13009S, Cell Signaling Technology, Danvers, MA, United States), rabbit anti-p-FAK (Tyr397, Cat No: 8556S, Cell Signaling Technology), rabbit anti-p-NF-κB (Ser536, Cat No: 3033L, Cell Signaling Technology), rabbit anti-ERK (Cat No: 9102S, Cell Signaling Technology), rabbit anti-p-ERK (Thr202/Tyr204, Cat No:9101S, Cell Signaling Technology), rabbit anti-JNK (Cat No: MBS8509129, MyBioSource, San Diego, CA, United States), rabbit anti-p-JNK (Thr183/Tyr185, Cat No: MBS8508944, MyBioSource), rabbit anti-P38 (Cat No: 9212S, Cell Signaling Technology), rabbit anti-p-P38 (Cat No: 9211S, Cell Signaling Technology), rabbit anti-TLR4 (Cat No: PA5-11597, Thermo Scientific, Waltham, MA, United States), and rabbit anti-TLR4 (Cat No: NB100-56566, Novus Biologicals, Littleton, CO, United States). Horseradish peroxidase (HRP)-conjugated anti-mouse (Cat No: ADI-SAB-100-J, Enzo Life Science, NY, United States), and rabbit IgG (Cat No: ADI-SAB-300-J, Enzo Life Science, NY, United States) were used as the secondary antibodies. LPS from Escherichia coli O111:B4 (Cat No: L2880, Sigma, St. Louis, MO, United States) and the TLR4 inhibitor TAK-242 were purchased from Calbiochem (Cat No: 614316, San Diego, CA, United States), and the p-FAK inhibitor PF-573228 (Cat No: S2031) was purchased from Selleckchem (Houston, TX, United States).