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Formaldehyde crosslinking assays were performed as previously described [65]. Trypsinized cells were resuspended and incubated in a 1.0–1.5% formaldehyde solution at room temperature for 30 min. Then, the reaction was quenched by the addition of a 1.5 M glycine solution to a final concentration of 0.15 M and incubated at room temperature for 5 min. Cells were pelleted by centrifugation at 4000 rpm at room temperature for 5 min and resuspended and washed twice in PBS. Products were analysed by western blot using PCNA (PC10) or Myc-tagged antibodies.
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Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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