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Extraction and measurement of anthocyanins
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The aerial parts of 10 day old Arabidopsis seedlings were collected, and then lyophilized and measured for dry weight. Total anthocyanins were extracted using 1 μL extraction buffer (50% methanol containing 3% formic acid) per 50 μg dry weight. After overnight incubation with extraction buffer at room temperature, the supernatant was collected and measured absorbance of 532 nm.
Copyright and License information: ©2019, Cao et al
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