Effects of Drought and ABA on Seed Germination

Wild-type (WT) and T3 homozygous transgenic seeds were surface sterilized, kept at 4°C for 3 days, and then sown on a germination medium. For the germination assay, seeds were placed on the germination medium with 6% or 10% (w/v) polyethylene glycol (PEG6000) 6000 (to simulate osmotic stress) and 0.5 μM or 1 μM ABA. After 3 days of vernalization, the seeds were transferred to normal conditions for germination. The percentage of germinated seeds was calculated based on the number of seedlings at 12, 24, 36, 48, 60, and 72 h. To obtain reproducible results, each experiment was repeated three times.