Mouse MM and UB Cell Culture

Mouse UB and MM (CMUB-1, CMMM-1; Probetex, Inc., San Antonio, TX) and MM cells deficient of PDGF receptor (PDGFR)-β null (−/−)^{17, 18} were characterized, grown, and maintained at 37°C in 5% CO₂ in Dulbecco's Modified Eagle's Medium (DMEM) that contained 10% fetal bovine serum as originally described.^{11, 12, 17} The cells were grown to confluence, underwent cell dissociation with trypsin, and washed for subsequent growth in monolayer and 3D culture as described below. PDGF-BB and VEGF-A (R&D Systems, Minneapolis, MN) were used at concentrations previously used by our laboratory or known to induce vasculogenesis in culture (Cell Proliferation).