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O eeRo was solubilized in a solution of ethanol and water 1:1, this solution was added separately to dichloromethane, ethyl acetate and buthanol, at different times of fractionation, in a separator funnel to get DCM, EA and ButOH fractions, respectively according to Kamdem et al., 2012 with some modifications.10
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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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