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Twelve DIV primary cortical neurons were treated with NMDA 100 μM for 5 or 24 h. At the end of the treatment, the medium was collected and the release of lactate dehydrogenase (LDH) in the medium was quantified to assess cell viability using the Cytotoxic 96 non-radioactive cytotoxicity assay kit (Promega, WI, USA).
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