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CEllular thermal shift assay (CETSA)
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CETSA was performed as described46. Each sample was suspended in PBS supplemented with protease inhibitors after they had been washed in PBS. Samples were at 53 °C for 3 min, incubated at room temperature for 3 min, and then snap-frozen in liquid nitrogen. Samples were freeze-thawed twice and centrifuged at 20,000 g for 20 min at +4 °C and supernatants analyzed by immunoblotting.
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