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Flow cytometry analysis and fluorescence-activated cell sorting

WD Wenjing Du
LN Lan Ni
BL Baojun Liu
YW Ying Wei
YL Yubao Lv
SQ Sujing Qiang
JD Jingcheng Dong
XL Xijun Liu
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PC9 cells were labeled with anti-CD44-fluorescein isothioyanate (FITC) specific mouse antibody and isotype-matched mouse immunoglobins served as negative controls (BD Bioscience). Dead cells and debris were first gated out using Propidium iodide (PI) based on cell size and internal complexity. At least 10,000 cells were selected for each analysis. Under sterilized conditions, cells labeled by anti-CD44-fluorescein isothioyanate (FITC) were sorted by BD FACS cell sorter. The cell viability was determined using Annexin V/PI Apoptosis Detection kit (BD Biosciences, U.S.). All data were analyzed using Flowjo software24.

Copyright and License information: The Author(s) ©2018
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