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The MALT1 cleavage assay was performed with 500 nM or 10 µM of BCL10-MALT1 filaments, GST-BCL10-MALT1 species, and MALT1 V81R mutant. Protein samples were incubated in 384-well non-binding microplates and 20 μM of the MALT1 substrate Ac-LRSR-AMC was added. After 2 min incubation at 30 °C, the fluorescence of cleaved AMC was measured over 1 h by a Synergy 2 Microplate Reader (Biotek, US). The MALT1 protease activity is represented in relative fluorescence units and data from at least three independent experiments were used.
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