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Samples and resequencing

LC Lin Cheng
KK Kyu-Won Kim
YP Yong-Jin Park
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A heuristic set containing 358 rice accessions with 3 types of accessions (landraces, weedy, and bred) previously generated from worldwide varieties collected from the National GeneBank of the Rural Development Administration (RDA-Genebank, Republic of Korea) using the program PowerCore46 was selected for whole-genome resequencing47. In addition, 54 wild rice accessions were obtained from the International Rice Research Institute (IRRI) in 2017.

For the 358 Asian rice and 54 wild rice accessions from our database, plants were planted in a soft field with enough water. After checking the heading date (approximately 13 days), young leaves were sampled from one plant and stored at –80 °C prior to genomic DNA extraction using the DNeasy Plant Mini Kit (Qiagen). Qualified DNA was used for whole-genome resequencing of the collected rice varieties with an average coverage of approximately 16X on the Illumina HiSeq. 2000 Sequencing Systems Platform.

Copyright and License information: The Author(s) ©2019
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