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Wheat germ agglutinin (WGA) staining
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Cryosections were rinsed 3 times in PBS and incubated with a primary antibody against WGA conjugated to Alexa Fluor 488 (50 μg/ml, Invitrogen, CA) for 1 h at room temperature. Slides were then rinsed in PBS and DAPI staining was performed for 10 min and mounted with Vectashield mounting medium (Vector Labs, CA). Stained tissues were imaged using a LSM 510 meta confocal microscope and images were processed using Photoshop CS6 software.
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