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Determination of cytokine concentrations by ELISA
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Levels of IL-6 and TNF-α were determined in cell-free culture supernatants and in bronchoalveolar lavage (BAL) fluid from mice by R&D Systems ELISAs (Bio-Techne). IL-1β level in lung tissue homogenates was determined with a mouse eBioscience ELISA kit from Thermo Fisher Scientific.
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