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2.2. Adenoviral transduction in mouse liver cell line
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Mouse hepatic Hepa1–6 cells were transduced with the adenovirus expressing mouse Acsl1 shRNA (Ad-shAcsl1) or the control virus (Ad-shLacZ) at various multiplicity of infection (MOI) in 1 mL medium containing 0.5% FBS. After 5 h of infection, medium was replaced with fresh medium containing 10% FBS and cells were harvested 72 h post infection to isolate total RNA or cell lysates.
Copyright and License information: ©2018-12-20
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