ATP/ADP ratio measurements and neuronal survival

Neuronal somas expressing the ATP/ADP ratio sensor Perceval HR were excited using a 405‐nm diode laser and a 488‐nm line of an Argon laser, and emission was collected using a 494–553‐nm window (Vaarmann et al, 2016). Data are expressed as the ratio of fluorescence emission from neuronal soma evoked by 488 nm excitation/405 nm excitation (F488 nm/F405 nm).

In neuronal survival experiments, neurons expressing neuron‐specific pAAV‐hSyn‐DsRed1 were counted manually from 50 fields per dish by two observers.