Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

In utero statin treatment in mice

YO Yoshimi Okamoto-Uchida
RY Ruoxing Yu
NM Norio Miyamura
NA Norie Arima
MI Mari Ishigami-Yuasa
HK Hiroyuki Kagechika
SY Suguru Yoshida
TH Takamitsu Hosoya
MN Makiko Nawa
TK Takeshi Kasama
YA Yoichi Asaoka
RA Reiner Wimmer Alois
UE Ulrich Elling
JP Josef M. Penninger
SN Sachiko Nishina
NA Noriyuki Azuma
HN Hiroshi Nishina
request Request a Protocol
ask Ask a question
Favorite

Pregnant ICR mice (Nihon SLC) were reared under a normal 12 h light/dark cycle. The day of insemination was designated as embryonic day (E) 0. One mM ATV or 1 mM ATV plus 100 mM MVA diluted in PBS was injected into the left and right sides of the uteri at E5.5 (150 μl per side). Control mice were injected with 1% DMSO/PBS. At E10.5, the injected mice were sacrificed, and uteri and morphological changes in the embryos were examined under a light microscope.

Copyright and License information:  ©2016, The Author(s)
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A