Differentiated podocytes were grown on laminin-coated glass cover slips and then fixed in 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 1% bovine serum albumin, and stained with Phalloidin (Molecular Probes, USA). Cells were observed using a confocal laser scanning microscope (Leica, Germany).
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