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Total RNA was isolated from cells using Trizol (Thermo Fisher Scientific). Reverse-transcription and quantitative PCR (qPCR) were carried out with a two-step Platinum SYBR green qPCR SuperMix UDG kit (Thermo Fisher Scientific). Mastercycler (Eppendorf, Hamburg, Germany) was used for qPCR analysis. Primers were obtained from Thermo Fisher Scientific (sequences listed in Table 1). Target mRNA was normalized to β-actin.
Sequences of primers for real-time qPCR assay used in the study
Copyright and License information: ©2018 Han et al. This work is published and licensed by Dove Medical Press Limited
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