In-vitro growth curves assessing the effect of PEA on bacterial growth

Freshly prepared bacteria from a cryo-conserved aliquot were added to different tubes containing brain heart infusion and (i) PEA 0.01 μg/ml, (ii) PEA 0.1 μg/ml, (iii) PEA 1 μg/ml, and (iv) 0.017% DMSO (same amount of DMSO as in tubes with PEA 0.01, 0.1, and 1 μg/ml). Each condition was tested in triplicates. Tubes were incubated at 37°C under rotation (100 rpm). At different time points, the number of viable bacteria was assessed by performing 10-fold dilutions in 0.9% NaCl and plating on sheep blood agar. Viable bacteria were quantified after overnight incubation at 37°C.