Cell viability assay

Cell viability was measured using the MTT reagent (Calbiochem) and different doses of docetaxel. 5000 cells were plated onto a 96-well tissue culture plate and incubated at 37 °C for 48 hours before drug treatment. Docetaxel was added from 0.001 nM to 100 nM and cells were incubated for 72 hours at 37 °C. Media was removed from the cells and replaced with MTT reagent (5 mg/ml in PBS) for 4 hr. Absorbance was measured at 600 nm using a micro-plate reader ELx8200 (Biotek Instruments). Viability was the mean percentage of absorbance relative to an untreated well for three biological replicates.